Exhibit 23.1
CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM
We hereby consent to the incorporation by reference in this Registration Statement on Form S-3 of (i)
our report dated March 7, 2005 relating to the financial statements, financial statement schedules,
managements assessment of the effectivess of internal control over financial reporting and the
effectivess of internal control over financial reporting, which appears in Gladstone Commercial
Corporations Annual Report on Form 10-K for the year ended December 31, 2004 and; (ii) our report
dated July 13, 2005 relating to the historical summary of revenue, which appears in Gladstone
Commercial Corporations Current Report on Form 8-K/A dated July 29, 2005. We also consent to the
reference to us under the heading Experts in such Registration Statement.
/s/ PricewaterhouseCoopers LLP
McLean, VA
September 30, 2005